INTRODUCTION: This study aims to use immunohistochemistry to compare histopathological findings and transforming growth factor beta 1 (TGF-b1) levels in polyp tissue in cases of nasal polyp with histopathological findings and TGF-b1 levels in the nasal mucosa in patients with obstructive nasal deformity.
METHODS: The study group consisted of 20 patients. The control group consisted to 20 patients scheduled for nasal surgery due to septum deviation. Edema, mixed infection cell infiltration, eosinophil leukocyte infiltration and squamous metaplasia development were studied from hematoxylin-eosin stained slides containing polyp and conchal mucosa tissue specimens collected from the study and control groups. These were then assessed semi-quantitatively. The presence or absence of TGF-b1 antibody and positive reaction in the mucosal epithelium, submucosal glands, fibroblasts and inflammatory cells were evaluated immunohistochemically.
RESULTS: A positive association was determined between eosinophilia and edema on tissue staining with hematoxylin and eosin in cases of nasal polyp, while no edema was observed in any cases in the control group. Comparison of the control and study groups on immunohistochemical (TGF-b1 positive reaction) staining revealed no significant difference between epithelium, gland and fibroblast staining. Immunohistochemical staining of inflammatory cells was more pronounced in the study group.
DISCUSSION AND CONCLUSION: We conclude that TGF-b1 may play a role together with other mediators, although not alone, in the pathophysiology of nasal polyp.